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By D. Perlman (Ed.)

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C. Olson and C. L. ), pp. 71-83. Plenum, New York. , and Suzuki, H. J . Ferment. Technol. 51, 895-903. , and Okamura, S. Annu. Meet. Agric. Chem. Soc. , Kyoto p. 233. , and Morisi, F. Agric. Biol. Chem. 39, 277-279. Martin, C . K. , and Perlman, D. Biotechnol. Bioeng. 18, 217-237. , and Furuichi, E. Annu. Meet. Agric. Chem. Soc. , Sapporo p. 58. Mohan, R. , and Li, N. N. (1975). Biotechnol. Bioeng. 17, 1137-1156. Mosbach, K . , and Larsson, P. 0. (1970). Biotechnol. Bioeng. 12, 19-27. , and Mosbach, R.

Deghenghi et al. (1967) Estrone Nocardia restrictus ATCC 14,887 Afonso et al. (1966) Equilin, equilenin + estrone Cmynebucterium simplex, Nocardia rubra Nocardiu sp. ATCC 19,170 Rakhit and Singh (1971) 6& 19-oxido-4-andros teneSih et al. 17-dione 3~-Acetoxy-5a-chloro(fluoro)Mycobacteria SP, 19-oxidosterols Denot et al. (1967) 5a-Bromo-GP-19-oxidoLee (1971) 3P-Acetoxy-5a-bromo-6P,19Nocardia sp. ATCC 19,170 oxidosterols androstane-3,17-dione 3a,5a-Cycloandrostan-17-one Mycobacterium phlei KNGSF 70 Van der Waard et al.

1974). This enzymic process also has some disadvantages for industrial production of the acid, since the procedure is carried out by batch incubation of a mixture of L-histidine and intact cells. To develop a more efficient method, the continuous production of urocanic acid was investigated using immobilized microbial cells having high L-histidine ammonia-lyase activity. Several microorganisms having high enzyme activity were immobilized in a polyacrylamide gel lattice. Achrornobacter liquidurn was found to show the highest activity after immobilization.

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