By Bernard Stanford Massey, Harrie S. Massey, H. B. Gilbody
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Additional info for Electronic and Ionic Impact Phenomena V
And Litt, G. J. (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J. Immunol. Methods 125, 279–285. 11. Shindler, K. S. and Roth, K. A. (1996) Double immunofluorescent staining using two unconjugated primary antisera raised in the same species. J. Histochem. Cytochem. 44, 1331–1335. 3 Quantum Dots for Multicolor Tumor Pathology and Multispectral Imaging Johbu Itoh and Robert Yoshiyuki Osamura Summary Quantum dots (QDs) are new nanocrystal semiconductor fluorophores consisting of a cadmium selenide core and zinc sulfide or cadmium sulfide shell.
Conventional narrow-band filters for fluorophores, such as Cy3 or FITC, can be used to visualize certain QDs. For example, a Cy3 filter set containing a 545 ± 30-nm excitation filter, a 570-nm long pass dichroic beamsplitter, and a 610 ± 75-nm emission filter would adequately allow the visualization of QD 605. The 610 ± 75-nm emission filter, while not centered on the 605-nm wavelength, would allow the passage of emitted light at 605 nm because of its relatively wide bandwidth (75 nm). Furthermore, a FITC filter set containing a 470 ± 40-nm excitation filter, a 500-nm long pass dichroic beamsplitter, and a 525 ± 40-nm emission filter would transmit fluorescence from both QD 525 and 565.
And Simon, S. M. (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 21, 47–51. 7. Qdot Streptavidin Conjugates User Manual. , Eugene, OR, pp. 1–14. pdf. 8. Bobrow, M. , Litt, G. , Shaughnessy, K. , Mayer, P. , and Conlon, J. (1992) The use of catalyzed reporter deposition as a means of signal amplification in a variety of formats. J. Immunol. Methods 150, 145–149. 9. van Gijlswijk, R. , Zijlmans, H. , et al. (1997) Fluorochromelabeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization.