Download Paper and Thin Layer Chromatography by Ivor Smith and J. W. T. Seakins (Eds.) PDF

By Ivor Smith and J. W. T. Seakins (Eds.)

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Alternatively a variety of full, half and quarter plates can be coated. If desired, a thin plastic sheet can be placed between the aluminium and 44 CHROMATOGRAPHIC T E C H N I Q U E S glass plates to simplify cleaning. On turning the side lever through 180° the side of the apparatus, which operates on a hinge, is brought up and over the edge of the glass plates whilst, at the same time, the base is raised up to press the plate edges to the undersides of the guide rails. Thus the plate tops form a single surface irrespective of their individual thicknesses.

Avirin and Avicel. These two materials are micro-crystalline celluloses, the former being a technical grade and the latter a pharmaeutical grade although both give very similar results. (6 > 7) Plates care THIN LAYER CHROMATOGRAPHY 43 prepared as described for cellulose and best results are obtained by homogenizing 8 g. Avicel with 43 ml. water and allowing overnight drying at room temperature although 80°C for 30 minutes is also satisfactory. For two-way runs, 20-30 minutes drying in a hood with a rapid airstream is sufficient and the plate is then free of smell.

4. These "Panglas" tanks also have pressed ridges at the upper edges to hold both troughs and anti-syphon rods so that the previously necessary devices for trough suspension are no longer required and only the minimum of internal glass fitments is now necessary. These tanks are also suitable for high voltage electrophoresis as described in Vol. 2. * The Unikit(6) was originally designed as a complete teaching apparatus for ascending and descending paper chromatography and electrophoresis (see Vol.

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